Extraction of DNA from TRIZOL preparations: Difference between revisions
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== '''DNA extraction''' == | == '''DNA extraction''' == | ||
=== You will need: === | === You will need: === | ||
====Back Extraction Buffer==== | ==== 1. Back Extraction Buffer==== | ||
Containing 4 M Guanidine Thiocyanate (FW 118.16), 50 mM Sodium Citrate NaCi (FW 294.1) and 1 M Tris base (FW 121.14), prepared in ddH2O and sterile filtered. | Containing 4 M Guanidine Thiocyanate (FW 118.16), 50 mM Sodium Citrate NaCi (FW 294.1) and 1 M Tris base (FW 121.14), prepared in ddH2O and sterile filtered. | ||
For 25 mL, combine 11.82 g Guanidine Thiocyanate, 0.37 g Sodium Citrate NaCi and 3.03 g Tris base. Be careful to add small volumes of water, gradually, as the reagents will come up to the required volume VERY quickly. | For 25 mL, combine 11.82 g Guanidine Thiocyanate, 0.37 g Sodium Citrate NaCi and 3.03 g Tris base. Be careful to add small volumes of water, gradually, as the reagents will come up to the required volume VERY quickly. | ||
====Isopropanol==== | ==== 2. Isopropanol==== | ||
====70% Ethanol==== | ==== 3. 70% Ethanol==== | ||
====Qiagen Elution Buffer (or similar TE buffer)==== | ==== 4. Qiagen Elution Buffer (or similar TE buffer)==== | ||
=== Extraction steps === | === Extraction steps === | ||