Extraction of DNA from TRIZOL preparations: Difference between revisions
This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been completely removed |
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=== Extraction steps === | === Extraction steps === | ||
#Add 500 uL of Back Extraction Buffer (BEB) for every 1 mL of TRIZOL used in the initial extraction. Place on shaker for 10 min. | #Add 500 uL of Back Extraction Buffer (BEB) for every 1 mL of TRIZOL used in the initial extraction. Place on shaker for 10 min. Always mix by inversion or use the shaker. NEVER vortex samples, as it will destroy the DNA. | ||
#Centrifuge tubes at 12,000 G for 30 min at room temperature. | #Centrifuge tubes at 12,000 G for 30 min at room temperature. | ||
##Note: After this step is complete, set the centrifuge to cool to 4 deg C. | ##Note: After this step is complete, set the centrifuge to cool to 4 deg C. | ||