Difference between revisions of "High Fat Diet Treatment"
From Bridges Lab Protocols
Davebridges (Talk | contribs) (added note about ordering C57BL/6J mice) |
Davebridges (Talk | contribs) (updated caloric content of HFD) |
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| Research Diets | | Research Diets | ||
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| − | | | + | | 4.73 kcal/g |
| 45% | | 45% | ||
| 20% | | 20% | ||
Revision as of 10:43, 10 April 2014
To place mice on a high fat diet, here is the general protocol:
For C57BL/6J mice, order so that they are 9 weeks of age on the monday of delivery. Start the diet on the Wednesday (10 days later).
Diet Choices
For more information about diets see research diets
| Food | Vendor | Code | Caloric Content | Fat | Protein | Sucrose | Starch | Notes |
|---|---|---|---|---|---|---|---|---|
| Normal Chow Diet | Lab Diet | 5L0D | 3.02 kcal/g | 5% | 24% | 3.7% | 32% | Provided by ULAM |
| High Fat Diet | Research Diets | D12451 | 4.73 kcal/g | 45% | 20% | 17% | 7% | Pink |
| Control Diet (High Sucrose) | Research Diets | D12450B | 3.85 kcal/g | 10% | 20% | 35% | 31% | Yellow |
| Control Diet (High Starch) | Research Diets | D12450H | 3.85 kcal/g | 10% | 20% | 17% | 44% | Peach |
Treatment Protocol
- Start when mice are 10 weeks of age.
- For High Fat Diet mice place in cages 4 mice/cage as early as possible. Can us 5 mice/cage for normal chow/control diets.
- Re-cage (and ear tag mice if necessary) mice and replace food with ~400g food in the container. Record this weight.
- This food should last 2 weeks.
- Weigh mice at ~10 AM either weekly or bi-weekly and re-weigh food to determine food intake. Top up to 400g.
- Generally treatment lasts 12 weeks.
