Difference between revisions of "BDNF PCR"
From Bridges Lab Protocols
(Created page with "'''BDNF Primers''' ---- * oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common) * oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse) * oIMR01694 --- GGG AAC TTC CTG ACT AG...") |
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Latest revision as of 14:13, 1 April 2014
BDNF Primers
- oIMR0133 ---- ATG AAA GAA GTA AAC GTC CAC (Common)
- oIMR0113 ---- CCA GCA GAA AGA GTA GAG GAG (WT Reverse)
- oIMR01694 --- GGG AAC TTC CTG ACT AGG GG (Reverse)
BDNF Cycler Program
- 94C - 10 minutes (HotStart Taq)
- 94C - 30 seconds
- 55C - 30 seconds
- 72C - 30 seconds ----- Repeat steps 2-4 for 35 cycles
- 72C - 2 minutes
- 4C - Hold
