Rapamycin Injections: Difference between revisions

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 ==Preparation==
-*Weight out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube.  Bring up to 40 mL with water and filter by steriflip.  This is 5.2% PEG/Tween
+*Weigh out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube.  Bring up to 40 mL with water and filter by steriflip.  This is 5.2% PEG/Tween
-*Dissolve rapamycin at 20 mg/mL in water.
+*Dissolve rapamycin at 20 mg/mL (0.0219 M) in ethanol (prepare as a 1 mL aliquot and store at -20 C).
-*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (use this for 1 week).
+*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (109 uM; use this for 1 week).
-*Inject 10 uL/g body weight
+*IP inject 10 uL/g body weight (1 mg/kg)
 ==Alternate Rapamycin Injection Regimen==