Bradford Assay: Difference between revisions
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==Protocol== | ==Protocol== | ||
Cuvette Bradford Assay | |||
#Dilute reagent 5X in water, stable for 2-3 weeks | #Dilute reagent 5X in water, stable for 2-3 weeks | ||
#Pipet 1 mL into disposable plastic cuvette | #Pipet 1 mL into disposable plastic cuvette | ||
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##Blank then measure samples, absorbance must be less than 0.9 | ##Blank then measure samples, absorbance must be less than 0.9 | ||
##Print (hit Recall, then enter, then print) and attach to experiment | ##Print (hit Recall, then enter, then print) and attach to experiment | ||
Protein Lysate Bradford Assay | |||
#Dilute reagent 5X in water, stable for 2-3 weeks | |||
#In a 96 well plate, dilute sample 20X (190ul Reagent, 10ul Sample) | |||
#Add 5ul of sample to 100ul of reagent in well | |||
#Run "Bradford Assay Protocol" on Plate Reader | |||
==Reference== | ==Reference== | ||
*Wikipedia: [[wikipedia:Bradford_protein_assay|Bradford Protein Assay]] | *Wikipedia: [[wikipedia:Bradford_protein_assay|Bradford Protein Assay]] | ||
*PMID 942051 | *PMID 942051 | ||