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→Protocol: Master Mix
==Protocol==
Use the following Volumes per 50ul Reaction:
#10X GoTaq Buffer: 4 uL of 5X Go-Taq buffer 5uL ("Molecular Biology Stuff" box in freezer) #Forward PrimerMix: 0.4ul#Reverse Primer: 0.4ul5ul#dNTPs: 0.4uL 5uL of 2 10 mM ("Molecular Biology Stuff" box in freezer)#Sterile water: 13.6 uL 29ul #Polymerase Go-Taq: 1 uL 0.125uL (6th floor in Genotype Yellow Box "Molecular Biology Stuff" box in freezer)
#Template: 1 uL
Master Mix
#10X GoTaq Buffer: 50uL ("Molecular Biology Stuff" box in freezer)
#Primer Mix: 50ul
#dNTPs: 5uL of 10 mM ("Molecular Biology Stuff" box in freezer)
#Sterile water: 290ul
#Polymerase Go-Taq: 1.25ul ("Molecular Biology Stuff" box in freezer)
*Add Template Individually
Run PCR Program (approx 2 hours).
