Difference between revisions of "Transfer Buffer"

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(copied recipe from saltiel lab buffers)
 
m (10X Transfer Buffer: Changed from 10L total to 4L total)
 
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{| border="1"
 
{| border="1"
! Component || Weight per 10 L || Catalog Number
+
! Component || Weight per 4 L || Catalog Number
 
|-  
 
|-  
|Tris Base || 303 g || Sigma T6066-5KG
+
|Tris Base || 121.2 g || Sigma T6066-5KG
 
|-  
 
|-  
|Glycine || 1441 g || Sigma G7126-5KG
+
|Glycine || 576.4 g || Sigma G7126-5KG
 
|}
 
|}
 
[[ Category:Buffer ]]
 
[[ Category:Buffer ]]
 
[[ Category:Western Blotting ]]
 
[[ Category:Western Blotting ]]

Latest revision as of 19:34, 11 July 2013

10X Transfer Buffer

To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:

  • 100 mL 10X Buffer
  • 200 mL Methanol
  • 700 mL Water
Component Weight per 4 L Catalog Number
Tris Base 121.2 g Sigma T6066-5KG
Glycine 576.4 g Sigma G7126-5KG