GoTaq PCR Genotyping: Difference between revisions
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==Materials== | ==Materials== | ||
*GoTaq Master Mix | *GoTaq Master Mix | ||
*Primer Mix ( | *Primer Mix (from 100uM Primer Stocks): Add 10uL of forward and reverse primer, 20uL total, into 980uL of dH20 to make a 10uM solution. | ||
*Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template | *Reaction Mixture: 25uL GoTaq Mix, 10uL of Primer Mix, 14uL Nuclease-Free Water, and 1uL of template | ||
*Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr. | *Gel: 0.3g Agarose to 30mL of TAE, microwave until dissolved and add 1uL of EtBr. | ||