Glucose Tolerance Test: Difference between revisions
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==Materials (Bring | ==SOP== | ||
*[[SOP - Needle Capping]] | |||
*[[Safety and Animal Training]] | |||
==Materials (Bring to Procedure Room)== | |||
*Glucometer - AccuChek Advantage | *Glucometer - AccuChek Advantage | ||
*Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]] | *Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]]. collect in university hospital building, floor B2 in room B2F408 dock 5 across from central pharmacy. | ||
*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
*Syringes | *Syringes | ||
*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | *10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | ||
*If doing an oral glucose tolerance test need a gavage needle | |||
*Timer | *Timer | ||
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#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
#Bring mice to procedure room. Put water bottle on cage. | #Bring mice to procedure room. Put water bottle on cage. | ||
#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized | #Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood. | ||
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test. | ||
##Immobilize mouse and restrain tail with one hand | ##Immobilize mouse and restrain tail with one hand | ||
##Aim needle between the midline and the hip bone | ##'''For ipGTT''' Aim needle between the midline and the hip bone | ||
##Insert syringe (do not inject) into cavity | ##'''For oGTT''' Gently inert gavage needle into mouth | ||
##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | ##Insert syringe (do not inject) into cavity/mouth | ||
##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | |||
##Eject syringe. | ##Eject syringe. | ||
#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein | #At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein | ||
[[Category:Animal Work]] | |||
[[Category:Mouse Work]] | [[Category:Mouse Work]] | ||
[[Category:Glucose Homeostasis]] | [[Category:Glucose Homeostasis]] | ||
[[Category:Injections]] | [[Category:Injections]] | ||