Glucose Tolerance Test: Difference between revisions

Line 17:

#Remove food from mice for about 6h. Add do not feed tag to cages.

#Bring mice to procedure room. Put water bottle on cage.

#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized ~~capilary~~ about 2/3 full with blood.

#Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood.

#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).

#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.

#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test.

##Immobilize mouse and restrain tail with one hand

##Aim needle between the midline and the hip bone

##'''For ipGTT''' Aim needle between the midline and the hip bone

##Insert syringe (do not inject) into cavity

##'''For oGTT''' Gently inert gavage needle into mouth

##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over

##Insert syringe (do not inject) into cavity/mouth

##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over

##Eject syringe.

#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein

#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein

[[Category:Mouse Work]]

[[Category:Glucose Homeostasis]]

[[Category:Injections]]

@@ Line 17: / Line 17: @@
 #Remove food from mice for about 6h.  Add do not feed tag to cages.
 #Bring mice to procedure room. Put water bottle on cage.
-#Weigh mice, mark tails as necessary and take fasting glucose measurement.  If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capilary about 2/3 full with blood.
+#Weigh mice, mark tails as necessary and take fasting glucose measurement.  If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capillary about 2/3 full with blood.
 #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL).
-#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse.
+#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse or the mouth if doing an oral glucose tolerance test.
 ##Immobilize mouse and restrain tail with one hand
-##Aim needle between the midline and the hip bone
+##'''For ipGTT''' Aim needle between the midline and the hip bone
-##Insert syringe (do not inject) into cavity
+##'''For oGTT''' Gently inert gavage needle into mouth
-##Pull up syringe and ensure that only air is aspirated.  If urine or blood appears in the syringe, start over
+##Insert syringe (do not inject) into cavity/mouth
+##'''For ipGTT:''' Pull up the syringe and ensure that only air is aspirated.  If urine or blood appears in the syringe, start over
 ##Eject syringe.
-#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein
+#At desired intervals (normally 15, 30, 45, 60, 75, 90, and 120 min), take blood glucose measurements from the tail vein
 [[Category:Mouse Work]]
 [[Category:Glucose Homeostasis]]
 [[Category:Injections]]