Glucose Tolerance Test: Difference between revisions
added glucose strip ordering information |
added link to fasting insulin |
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#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
#Bring mice to procedure room. Put water bottle on cage. | #Bring mice to procedure room. Put water bottle on cage. | ||
#Weigh mice, mark tails as necessary and take fasting glucose measurement. | #Weigh mice, mark tails as necessary and take fasting glucose measurement. If necessary also take blood for [[Measuring Fasting Insulin]] by filling a heparinized capilary about 2/3 full with blood. | ||
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | ||