Glucose Tolerance Test: Difference between revisions
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added glucose strip ordering information |
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==Materials (Bring Downstairs)== | ==Materials (Bring Downstairs)== | ||
*Glucometer - AccuChek Advantage | *Glucometer - AccuChek Advantage | ||
*Glucose Test Strips - AccuChek Comfort Curve | *Glucose Test Strips - AccuChek Comfort Curve. Order these through Materiels Service by calling 936-6077 and ordering product 2535. For information see [[https://www31.med.umich.edu/materielsvcs/catalog/details.cfm?stock=2535 here]] | ||
*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
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#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||
#At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | #At 1 min intervals, inject appropriate amount of glucose into interperitoneal cavity of the mouse. | ||
##Immobilize mouse and restrain tail with one hand | |||
##Aim needle between the midline and the hip bone | |||
##Insert syringe (do not inject) into cavity | |||
##Pull up syringe and ensure that only air is aspirated. If urine or blood appears in the syringe, start over | |||
##Eject syringe. | |||
#At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein | #At desired intervals (normally 15, 30, 45, 60, 75, 90 and 120 min), take blood glucose measurements from tail vein | ||