Difference between revisions of "Genotyping Program"

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(added newer genotyping strains)
(added mc4r-Cone)
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!| Allele || Program|| Fragment Size(s) || Notes
 
!| Allele || Program|| Fragment Size(s) || Notes
 
|-  
 
|-  
|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || || Two bands if heterozygote
+
|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || Two bands if heterozygote
 
|-  
 
|-  
 
|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
 
|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
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|-  
 
|-  
 
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
 
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
|-
+
|
 
|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
 
|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
 
|-  
 
|-  
|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575, WT-714 || Separate Reactions for WT and KO
+
|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575bp, WT-714bp || Separate Reactions for WT and KO
 
|-
 
|-
 
|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote, upper band for KO
 
|[[CAP Genotyping|CAP]] || CAP ||  || Two bands if heterozygote, upper band for KO
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|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
 
|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
 
|-
 
|-
|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300, WT- 247 || Two bands if heterozygote
+
|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300bp, WT- 247bp || Two bands if heterozygote
 +
|-
 +
|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540, WT- 400 || Two bands if heterozygote (run primers separately
 
|}
 
|}
 +
 +
 
[[ Category:Genotyping ]]
 
[[ Category:Genotyping ]]
 
[[ Category:Mouse Work]]
 
[[ Category:Mouse Work]]

Revision as of 15:21, 5 June 2020

Allele Program Fragment Size(s) Notes
TSC-floxed Cre PCR KO- 368bp, WT- 295bp Two bands if heterozygote
Cre Cre PCR Only present if Transgene Positive (cannot detect homozygosity at transgene)
Vac14 Cre PCR Two bands if heterozygote
TC10b TC10b KO - 500bp, WT-1kb Two bands if heterozygote ob/ob Genotyping ob/ob KO - 123bp, WT-104kb, control 191 bp Two reactions, see ob/ob Genotyping
MTM1 MTM1 Mutant-575bp, WT-714bp Separate Reactions for WT and KO
CAP CAP Two bands if heterozygote, upper band for KO
Vinexin Vinexin
GR KO nr3c1 KO- 300bp, WT- 247bp Two bands if heterozygote
mc4r-Cone mc4r KO- 540, WT- 400 Two bands if heterozygote (run primers separately