High Fat Diet Treatment: Difference between revisions
filled in details about mouse diets |
update diet table |
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[[ Category: Diet ]] | |||
[[ Category: Animal Studies ]] | |||
[[ Category: Glucose Homeostasis ]] | |||
[[ Category: Metabolism ]] | |||
[[ Category: Mouse Work ]] | |||
To place mice on a high fat diet, here is the general protocol: | To place mice on a high fat diet, here is the general protocol: | ||
For C57BL/6J mice, order so that they are 9 weeks of age on the monday of delivery. Start the diet on the Wednesday (10 days later). | |||
==Diet Choices== | ==Diet Choices== | ||
For more information about diets see [http://www.researchdiets.com/system/resources/BAhbBlsHOgZmIisyMDEyLzA0LzIwLzE0XzM5XzQwXzMxN19ESU9fU2VyaWVzLnBkZg/DIO%20Series.pdf research diets] | For more information about diets see [http://www.researchdiets.com/system/resources/BAhbBlsHOgZmIisyMDEyLzA0LzIwLzE0XzM5XzQwXzMxN19ESU9fU2VyaWVzLnBkZg/DIO%20Series.pdf research diets] | ||
for caloric content in future diets, use metabolizable energy, not physiological fuel value. Metabolizable energy is more accurate as it is derived from bomb calorimetry | |||
{| border=1 | {| border=1 | ||
| Line 21: | Line 30: | ||
| Lab Diet | | Lab Diet | ||
| 5L0D | | 5L0D | ||
| | | 2.91 kcal/g | ||
| 5% | | 5% | ||
| 24% | | 24% | ||
| Line 31: | Line 40: | ||
| Research Diets | | Research Diets | ||
| D12451 | | D12451 | ||
| | | 4.73 kcal/g | ||
| 45% | | 45% | ||
| 20% | | 20% | ||
| Line 54: | Line 63: | ||
| 10% | | 10% | ||
| 20% | | 20% | ||
| | | 17% | ||
| 44% | | 44% | ||
| Peach | | Peach | ||
|- | |||
| Control Diet (KD) | |||
| Research Diets | |||
| D1053001 | |||
| 3.8 kcal/g | |||
| 10% | |||
| 15% | |||
| 0% | |||
| 75% | |||
| Pellet form | |||
|- | |||
| Ketogenic Diet | |||
| Research Diets | |||
| D17053002 | |||
| 6.4 kcal/g | |||
| 85% | |||
| 15% | |||
| 0% | |||
| 0% | |||
| Paste form | |||
|} | |} | ||
==Treatment Protocol== | |||
# Start when mice are 10 weeks of age. | # Start when mice are 10 weeks of age. | ||