Bradford Assay: Difference between revisions

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Protein Lysate Bradford Assay
Protein Lysate Bradford Assay
#Dilute reagent 5X in water, stable for 2-3 weeks
#Dilute reagent 5X in water, stable for 2-3 weeks
#In a 96 well plate, dilute sample 20X (190ul Reagent, 10ul Sample)
#In a 96 well plate, dilute sample 20X (190ul H2O, 10ul Sample)--this dilution factor is tissue-dependent, only need to dilute fat ~5x
#Add 5ul of sample to 100ul of reagent in well
#Add 5ul of 20x diluted sample to either 100ul or 200ul of Bradford reagent in well.
##Prepare a standard curve by adding 0.0-3.0ul BSA std (1mg/ml) in increments of 0.5ul for 100 ul or 0-6ul BSA std in increments of 1ul for 200ul.
#Run "Bradford Assay Protocol" on Plate Reader
#Run "Bradford Assay Protocol" on Plate Reader