Bradford Assay: Difference between revisions
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Protein Lysate Bradford Assay | Protein Lysate Bradford Assay | ||
#Dilute reagent 5X in water, stable for 2-3 weeks | #Dilute reagent 5X in water, stable for 2-3 weeks | ||
#In a 96 well plate, dilute sample 20X (190ul | #In a 96 well plate, dilute sample 20X (190ul H2O, 10ul Sample)--this dilution factor is tissue-dependent, only need to dilute fat ~5x | ||
#Add 5ul of sample to 100ul of reagent in well | #Add 5ul of 20x diluted sample to either 100ul or 200ul of Bradford reagent in well. | ||
##Prepare a standard curve by adding 0.0-3.0ul BSA std (1mg/ml) in increments of 0.5ul for 100 ul or 0-6ul BSA std in increments of 1ul for 200ul. | |||
#Run "Bradford Assay Protocol" on Plate Reader | #Run "Bradford Assay Protocol" on Plate Reader | ||