Difference between revisions of "ChIP Elution Buffer"
From Bridges Lab Protocols
(Created page with "10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.") |
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| + | ! Chemical !! Final Concentration !! Per 5mL !! Stock !! Location | ||
| + | |- | ||
| + | | SDS || 1% || 500uL || 10% || Solutions Shelf | ||
| + | |- | ||
| + | | NaHCO3 || 0.1M || 42g || Powder || Chemical Shelf | ||
| + | |} | ||
| + | |||
| + | OR you can make this way but the stocks for this are not made up, it ends up being the same final concentrations | ||
10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water. | 10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water. | ||
Latest revision as of 22:09, 9 January 2018
| Chemical | Final Concentration | Per 5mL | Stock | Location |
|---|---|---|---|---|
| SDS | 1% | 500uL | 10% | Solutions Shelf |
| NaHCO3 | 0.1M | 42g | Powder | Chemical Shelf |
OR you can make this way but the stocks for this are not made up, it ends up being the same final concentrations 10 μL 20% SDS, 20 μL 1 M NaHCO3 and 170 μL sterile, distilled water.
