Chromatin Immunoprecipitation: Difference between revisions
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28. Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction: | 28. Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction: | ||
** [[Low Salt Immune Complex Wash Buffer]] (Catalog # 20-154), one wash | ** [[Low Salt Immune Complex Wash Buffer]] (Catalog # 20-154), one wash | ||
** High Salt Immune Complex Wash Buffer (Catalog # 20-155), one wash | ** [[High Salt Immune Complex Wash Buffer]] (Catalog # 20-155), one wash | ||
** [[LiCl Immune Complex Wash Buffer]] (Catalog # 20-156), 3-5 washes | ** [[LiCl Immune Complex Wash Buffer]] (Catalog # 20-156), 3-5 washes | ||
** TE Buffer (Catalog # 20-157), two washes | ** TE Buffer (Catalog # 20-157), two washes | ||