Low Salt Immune Complex Wash Buffer: Difference between revisions

← Older edit

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 ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location
 |-
-| LiCL || 250mM || 3.75mL || 1M || Solutions Shelf
+| SDS || 1% || 150uL || 10% || Solutions Shelf
 |-
-| NP40 || 1% || 1.5mL || 10% || Solutions Shelf
+| Triton X-100 || 1% || 1.5mL || 10% || Solutions Shelf
 |-
-| NaDC || 1% || 1.5mL || 10% || Solutions Shelf
+| NaCl || 150mM || 563uL || 4M || Solutions Shelf
 |-
-| EDTA || 1mM || 30uL || 500mM || Solutions Shelf
+| EDTA || 2mM || 60uL || 500mM || Solutions Shelf
 |-
-| Tris || 10mM || 150uL || 1M || Solutions Shelf
+| Tris || 20mM || 300uL || 1M || Solutions Shelf
 |}