Bridges Lab Protocols

Low Salt Immune Complex Wash Buffer: Difference between revisions

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Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex..."
 
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! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location
! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location
|-
|-
| LiCL || 250mM || 3.75mL || 1M || Solutions Shelf
| SDS || 1% || 150uL || 10% || Solutions Shelf
|-
|-
| Example || Example || Example || Example || Example
| Triton X-100 || 1% || 1.5mL || 10% || Solutions Shelf
|-
|-
| Example || Example || Example || Example || Example
| NaCl || 150mM || 563uL || 4M || Solutions Shelf
|-
|-
| Example || Example || Example || Example || Example
| EDTA || 2mM || 60uL || 500mM || Solutions Shelf
|-
|-
| Example || Example || Example || Example || Example
| Tris || 20mM || 300uL || 1M || Solutions Shelf
|}
|}
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Low_Salt_Immune_Complex_Wash_Buffer"