Difference between revisions of "Low Salt Immune Complex Wash Buffer"
From Bridges Lab Protocols
(Created page with "{| class="wikitable" |- ! Chemical !! Final Concentration !! Per 15 mL !! Stock !! Location |- | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf |- | Example || Example || Ex...") |
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| LiCL || 250mM || 3.75mL || 1M || Solutions Shelf | | LiCL || 250mM || 3.75mL || 1M || Solutions Shelf | ||
|- | |- | ||
− | | | + | | NP40 || 1% || 1.5mL || 10% || Solutions Shelf |
|- | |- | ||
− | | | + | | NaDC || 1% || 1.5mL || 10% || Solutions Shelf |
|- | |- | ||
− | | | + | | EDTA || 1mM || 30uL || 500mM || Solutions Shelf |
|- | |- | ||
− | | | + | | Tris || 10mM || 150uL || 1M || Solutions Shelf |
|} | |} |
Revision as of 19:52, 9 January 2018
Chemical | Final Concentration | Per 15 mL | Stock | Location |
---|---|---|---|---|
LiCL | 250mM | 3.75mL | 1M | Solutions Shelf |
NP40 | 1% | 1.5mL | 10% | Solutions Shelf |
NaDC | 1% | 1.5mL | 10% | Solutions Shelf |
EDTA | 1mM | 30uL | 500mM | Solutions Shelf |
Tris | 10mM | 150uL | 1M | Solutions Shelf |