Chromatin Immunoprecipitation: Difference between revisions
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supernatant fraction. | supernatant fraction. | ||
# Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction: | # Wash the Protein G Agarose-antibody/chromatin complex by resuspending the beads in 1 mL each of the cold buffers in the order listed below and incubating for 3-5 minutes on a rotating platform followed by brief centrifugation (3000-5000 x g for 1 minute) and careful removal of the supernatant fraction: | ||
* Low Salt Immune Complex Wash Buffer (Catalog # 20-154), one wash | |||
* High Salt Immune Complex Wash Buffer (Catalog # 20-155), one wash | |||
* LiCl Immune Complex Wash Buffer (Catalog # 20-156), one wash | |||
* TE Buffer (Catalog # 20-157), two washes | |||
D. Elution of Protein/DNA Complexes | D. Elution of Protein/DNA Complexes | ||
Prior to starting this section: | Prior to starting this section: | ||