Difference between revisions of "Submitting Plasmids for Sequencing"
From Bridges Lab Protocols
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Revision as of 19:26, 19 October 2017
- Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul
- These should be submitted in 1.5mL tubes
- Go to https://client-seqcore.brcf.med.umich.edu/login.cgi?exp=1
- PI login: Bridges
- Dave must add you to the lab under his account, he will give you your username and the password needed
- Typically we will select Sanger Sequencing (the only low throughput option)
- Select 'Submit samples/plates'
- Select the appropriate short-code from the drop down menu at the top of this page
- Generally choose 'One-at-a-time Sample Submission' unless you have a lot
- From here enter the following for each section:
- The sample name (the sample name that mean something to you regarding the sequence)
- No discounts
- Mini-Prep Plasmid
- Whatever vector you used for this plasmid
- Size of the insert you are sequencing
- Custom Primers
- Do not save
- Do not rush
- Save and repeat with your other samples
- Go to ... for locations and drop off times
