Glucose Tolerance Test: Difference between revisions
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==Materials (Bring Downstairs)== | ==Materials (Bring Downstairs)== | ||
*Glucometer | *Glucometer - AccuChek Advantage | ||
*Glucose Test Strips | *Glucose Test Strips - AccuChek Comfort Curve | ||
*Scale | *Scale | ||
*Beaker for weighing mice | *Beaker for weighing mice | ||
*Syringes | *Syringes | ||
*10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | *10% Glucose in PBS (make as 1g in 10 mL, sterile filtered). This will correspond to 1g/kg glucose injections. | ||
*Timer | |||
==Protocol== | ==Protocol== | ||
#Remove food from mice for about 6h. Add do not feed tag to cages. | #Remove food from mice for about 6h. Add do not feed tag to cages. | ||
#Bring mice to procedure room. | #Bring mice to procedure room. Put water bottle on cage. | ||
#Weigh mice, mark tails as necessary and take fasting glucose measurement. | #Weigh mice, mark tails as necessary and take fasting glucose measurement. | ||
#Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | #Prepare glucose syringes with 10 uL per g mouse weight (ie for a 30g mouse, 300 uL). | ||