Chromatin Immunoprecipitation: Difference between revisions
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# Add 200 μl PBS/BSA (for each sample you plan to use this antibody with) to beads. | # Add 200 μl PBS/BSA (for each sample you plan to use this antibody with) to beads. | ||
# Add 1 μg primary antibody. Mix gently by tapping--Do not vortex beads after adding the antibody. | # Add 1 μg primary antibody. Mix gently by tapping--Do not vortex beads after adding the antibody. | ||
# Gently mix on a rotator platform for at least 2 hours at 4°C. | # Gently mix on a rotator platform for at least '''2 hours''' at 4°C. | ||
# Wash beads 3 times (steps b-c), resuspending the beads by inverting the tubes during each wash. | # Wash beads 3 times (steps b-c), resuspending the beads by inverting the tubes during each wash. | ||
# Resuspend in 100 μl PBS/BSA (for each sample you plan to use this antibody with), and proceed to Step 2. | # Resuspend in 100 μl PBS/BSA (for each sample you plan to use this antibody with), and proceed to Step 2. | ||