Preparation of Tail Samples (for Genotyping): Difference between revisions
m →Protocol: Proteinase K |
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==Protocol== | ==Protocol== | ||
# Combine | # Combine 100uL of PBND solution with 2ul of Proteinase K and mouse tail in an eppendorf tube or in a well of a 96 well PCR plate (Proteinase K stock = 10mg/mL) | ||
# Incubate at 55 degrees (O/N) | # Incubate at 55 degrees (16 hours - O/N) | ||
# Incubate at 85 degrees for | # Incubate at 85 degrees for 60 min | ||
# | # Hold at 4 degrees | ||
[[Category: Mouse Work]] | [[Category: Mouse Work]] | ||