Chromatin Immunoprecipitation: Difference between revisions
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# Collect beads containing immuno-bound chromatin by placing the microfuge tube on a magnet rack. | # Collect beads containing immuno-bound chromatin by placing the microfuge tube on a magnet rack. | ||
# Remove and discard supernatant. | # Remove and discard supernatant. | ||
# Wash beads 5 times with LiCl Wash Buffer, mixing 3 minutes for each wash on a rotator. | # Wash beads 5 times with 1 mL LiCl Wash Buffer, mixing 3 minutes for each wash on a rotator. | ||
# Add 1 ml TE Buffer to beads. Mix 1 minute on rotator and then place tubes on magnet rack to collect beads and discard supernatant. | # Add 1 ml TE Buffer to beads. Mix 1 minute on rotator and then place tubes on magnet rack to collect beads and discard supernatant. | ||
# Resuspend the bead pellet in 200 μl IP Elution Buffer (at room temperature). Vortex to mix. | # Resuspend the bead pellet in 200 μl IP Elution Buffer (at room temperature). Vortex to mix. | ||