View source for Adipocyte Cell Counting with ImageJ

==ImageJ Download==
*Download ImageJ from http://imagej.nih.gov/ij/download.html
**To to open ImageJ, right click on software in applications folder
***Since the program is downloaded from the internet it will not open up automatically and you must go through applications folder.
==Adipocyte Counting Macro==
*Download adipocyte counting tool from http://dev.mri.cnrs.fr/projects/imagej-macros/wiki/Adipocytes_Tool
*Open ImageJ
*Click on Plugins
**Macros
***Install
****Select "MRI_Adipocyte_Tools" from downloads to install.
==Adipocyte Cell Counting==
*With macro installed, click on segmentation: "s"
**Set boundaries for sizing of cell
***Use 40-40,000 for size typically.
**Change thresholding method to Default.
*Open image of interest.
*Click on segmentation "s"
*Select freehanding tool to outline any cells that were not counted
**To add freehanded cell to ROI manager, click command + T
*Once all cells have been outlined, and misnumbered/outlined cells have been removed, click on measure
**Copy and save measurements to Excel spreadsheet
===To save image of outlined cells===
*Make sure all cells are outlined.
*Click on "Flatten"
*Save flattened image to folder.