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Changes
→Crosslinking, Lysis and Shearing of DNA: specified tube for sonicaiton
10. Collect the crude nuclear prep by centrifuging at 2,000 rpm at 4°C for 5 minutes.
11. Resuspend pellet to 1 ml with RIPA Buffer in a 15 mL falcon tube (Do not vortex the tubes and try to avoid bubbles. Bubbles will cause popping and loss of samples during sonication).
12. Using the Sonics VibraCell Sonicator, sonicate each 1.0 ml ChIP sample on ice, in a cold room, at Power Output 5 watts 6 times for 30 seconds each
