915
edits
Changes
m
changed volume of polymerase
* Using Platinum Taq High Fidelity amplify region of interest (design primers, should be <1000bp):
* Per reaction:
** 2221.5 uL Platinum Taq High Fidelity Master Mix
** 2.5 uL of Primers from a 2 uM stock solution
** 1 uL of the Extracted DNA from the previous step.