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→NEFA determination from serum (use Wako Diagnostics NEFA-HR(2) Microtiter Kit)
== NEFA determination from serum (use Wako Diagnostics NEFA-HR(2) Microtiter Kit) ==
#To wells of a clear 96 well plate, add 4uL of water (as blank), appropriate standard (the kit comes with stock of 1 mEq/L. Dilute the stock in ddH20 to give 0.25 and 0.5 mEq/L standards. For the high standard, add 8 uL of the 1 mEq/L stock. Due to the extra volume, the concentration of this last standard is actually 1.97 mEq/L).
#To remaining wells, add 4 uL of serum samples. ***Note: You may need to add more/less serum, depending on the NEFA concentration of your samples***
