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Created page with "== Rehydrate tissue sections == #After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute ..."
== Rehydrate tissue sections ==
#After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute washes using fresh xylene each time
#Rehydrate the tissue sections by immersing in 100% ethanol for 3 minutes. Repeat a second time using fresh 100% ethanol
#Immerse sections in 95% ethanol for 3 minutes. Repeat a second time using fresh 95% ethanol
#Immerse sections in 70% ethanol for 3 minutes
#Rinse sections 2 to 3 times in distilled water
== Stain tissue sections with haematoxylin ==
#Immerse sections in pre-prepared Mayer's haematoxylin for 5 minutes
#Rinse under running tap water (slides still in box) until water is clear. This will take about 5 minutes
== Stain tissue sections with eosin, dehydrate and coverslip ==
#Immerse sections in 1% eosin from 15 seconds to 1 minute
#Rinse 3-4 times under running tap water
#Immerse in 70% ethanol for 2 minutes
#Immerse in in 95% ethanol for 2 minutes. Repeat a second time using fresh 95% ethanol
#Immerse in in 100% ethanol for 2 minutes. Repeat twice more using fresh 100% ethanol
#Immerse in xylene for 2 minutes. Repeat twice more using fresh xylene. Check staining under the microscope
#Coverslip using Permount or other xylene-based mounting medium
#After cutting sections of tissue onto glass slides, de-pariffinize the tissue sections in xylene for 3 min. Repeat for a total of 3x 3 minute washes using fresh xylene each time
#Rehydrate the tissue sections by immersing in 100% ethanol for 3 minutes. Repeat a second time using fresh 100% ethanol
#Immerse sections in 95% ethanol for 3 minutes. Repeat a second time using fresh 95% ethanol
#Immerse sections in 70% ethanol for 3 minutes
#Rinse sections 2 to 3 times in distilled water
== Stain tissue sections with haematoxylin ==
#Immerse sections in pre-prepared Mayer's haematoxylin for 5 minutes
#Rinse under running tap water (slides still in box) until water is clear. This will take about 5 minutes
== Stain tissue sections with eosin, dehydrate and coverslip ==
#Immerse sections in 1% eosin from 15 seconds to 1 minute
#Rinse 3-4 times under running tap water
#Immerse in 70% ethanol for 2 minutes
#Immerse in in 95% ethanol for 2 minutes. Repeat a second time using fresh 95% ethanol
#Immerse in in 100% ethanol for 2 minutes. Repeat twice more using fresh 100% ethanol
#Immerse in xylene for 2 minutes. Repeat twice more using fresh xylene. Check staining under the microscope
#Coverslip using Permount or other xylene-based mounting medium
