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Primer Design for qPCR

125 bytes added, 14:51, 31 July 2014
updated specifying to start with the ncbi gene page
==Making Your Own Primers From Entrez==
# Find your RNA sequence on entrez by going to starting with the gene at http://www.ncbi.nlm.nih.gov/nuccore gene (make sure its the correct species). Then pick the mRNA you want to probe, based on the isoform structure on that page. Click on that nucleotide
# Under analyse this sequence click '''Pick Primers'''
# Under PCR Product Size pick 70-150 as the range

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