915
edits
Changes
wrote page for making plates
==Materials==
* Petri Dishes
* Bacto Agar (BD 214010)
* LB Broth (Sigma L3522)
* Antibiotic, usually at 1000X, add 1 uL/mL of media
==Protocol==
* 500 mL makes about one sleeve of petri dishes
* Add 25g LB Broth per Liter to an appropriately sized beaker
* Add Water and Stir until clumps are gone
* Add 15 g Agar per Liter , this will not dissolve
* Transfer to a erlenmeyer flask at least 2x the volume that you are making
* Autoclave for at least a 20 minute liquid cycle
* Once the liquid is cool to the touch (dosent burn your hand to hold) add antibiotic and mix by swirling
* Pour into plates, covering the surface and avoiding bubbles
* Burn off bubbles with bunsen burner or pop with a sterile tip
* Let plates cool with the lids ajar
* Invert plates, place in sleeve and mark sleeve as LB/Amp or LB/Km depending on the antibiotic
[[ Category: Cloning ]]
[[ Category: Media ]]
[[ Category: Molecular Biology]]
* Petri Dishes
* Bacto Agar (BD 214010)
* LB Broth (Sigma L3522)
* Antibiotic, usually at 1000X, add 1 uL/mL of media
==Protocol==
* 500 mL makes about one sleeve of petri dishes
* Add 25g LB Broth per Liter to an appropriately sized beaker
* Add Water and Stir until clumps are gone
* Add 15 g Agar per Liter , this will not dissolve
* Transfer to a erlenmeyer flask at least 2x the volume that you are making
* Autoclave for at least a 20 minute liquid cycle
* Once the liquid is cool to the touch (dosent burn your hand to hold) add antibiotic and mix by swirling
* Pour into plates, covering the surface and avoiding bubbles
* Burn off bubbles with bunsen burner or pop with a sterile tip
* Let plates cool with the lids ajar
* Invert plates, place in sleeve and mark sleeve as LB/Amp or LB/Km depending on the antibiotic
[[ Category: Cloning ]]
[[ Category: Media ]]
[[ Category: Molecular Biology]]
