915
edits
Changes
wrote protocol for amitriptyline in water
[[Category:Mouse Work]]
[[Category:Animal Studies]]
[[Category:Drug Treatment]]
[[Category:Insulin Sensitivity]]
[[Category:Insulin Signaling]]
[[Category:Metabolism]]
We use Amitiptyline from Sigma (A8404).
==For drinking water administration ~10 mg/kg/day==
We have approval for 1-100 mg/kg/day. Still need to pilot these effects
* Makes 1L of drinking water
* dissolve 0.092g in 1L water
===Calculation===
* 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg Dex/kg/mL
* average mouse weighs 0.03kg so want 0.06 mg Dex/mL or 60 mg/L
*This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored.
[[Category:Animal Studies]]
[[Category:Drug Treatment]]
[[Category:Insulin Sensitivity]]
[[Category:Insulin Signaling]]
[[Category:Metabolism]]
We use Amitiptyline from Sigma (A8404).
==For drinking water administration ~10 mg/kg/day==
We have approval for 1-100 mg/kg/day. Still need to pilot these effects
* Makes 1L of drinking water
* dissolve 0.092g in 1L water
===Calculation===
* 10 mg drug/kg/day because mice drink ~5mL /day so want 2 mg Dex/kg/mL
* average mouse weighs 0.03kg so want 0.06 mg Dex/mL or 60 mg/L
*This assumes that the mice are about 30g and they drink 5mL/day. The volume drank per cage should be monitored.
