915
edits
Changes
added nots on how to make primer mixes and added band sizes
* ob/ob - WT-Lep-F: 5'- AAT GAC CTG GAG AAT CTC C -3'
* ob/ob - Lepob-R: 5'- GCA GAT GGA GGA GGT CTC A -3'
===Primer Mixes===
Primers are resuspended at 100 uM, so add 3x10 uL of the primers to 970uL of water to make the primer mixes:
*For '''WT Primers''' combine both RFLP primers with the WT-Lep-F primer
*For '''Ob Primers''' combine both RFLP primers with the Lebob-R primer
==PCR Reaction==
==Bands==
[[ Category:Genotyping ]]
