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#add 5 mL culture to 1L TB/Amp and grow at 37C
#grow to OD600 of 0.6-1.0 and induce with 10 uM IPTG (optimize for each protein)
#let grow O/N at <25C (optimize induction time/temp for each protein, see [[Induction Conditions]]).
#centrifuge cells 10 min at 9000 RPM to pellet bacteria freeze if stopping at this point
==Lysis and Purification==
