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copied recipe from saltiel lab buffers
==10X Transfer Buffer==
To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:
* 100 mL 10X Buffer
* 200 mL Methanol
* 700 mL Water
{| border="1"
! Component || Weight per 10 L || Catalog Number
|-
|Tris Base || 303 g || Sigma T6066-5KG
|-
|Glycine || 1441 g || Sigma G7126-5KG
|}
[[ Category:Buffer ]]
[[ Category:Western Blotting ]]
To make 1X TBS, dilute 10X with water, and add 20% methanol. For example:
* 100 mL 10X Buffer
* 200 mL Methanol
* 700 mL Water
{| border="1"
! Component || Weight per 10 L || Catalog Number
|-
|Tris Base || 303 g || Sigma T6066-5KG
|-
|Glycine || 1441 g || Sigma G7126-5KG
|}
[[ Category:Buffer ]]
[[ Category:Western Blotting ]]
