915
edits
Changes
wrote genotyping protocol from Ellet et al
Used method described in Ellet et al (http://dx.doi.org/10.1038/oby.2008.443)
==Primers==
* ob/ob RFLP-F: 5'- TGA GTT TGT CCA AGA TGG ACC -3'
* ob/ob RFLP-R: 5'- GCC ATC CAG GCT CTC TGG -3'
* ob/ob - WT-Lep-F: 5'- AAT GAC CTG GAG AAT CTC C -3'
==PCR Reaction==
The PCR program is called '''ob/ob''' and is:
* 95 °C 2 min
* 40 cycles of:
** 95 °C 30 s
** 58 °C 30 s
** 72 °C 45 s
*72 °C 7 min.
==Primers==
* ob/ob RFLP-F: 5'- TGA GTT TGT CCA AGA TGG ACC -3'
* ob/ob RFLP-R: 5'- GCC ATC CAG GCT CTC TGG -3'
* ob/ob - WT-Lep-F: 5'- AAT GAC CTG GAG AAT CTC C -3'
==PCR Reaction==
The PCR program is called '''ob/ob''' and is:
* 95 °C 2 min
* 40 cycles of:
** 95 °C 30 s
** 58 °C 30 s
** 72 °C 45 s
*72 °C 7 min.
