Changes

Jump to: navigation, search

Lipofectamine Plasmid Transfection

65 bytes added, 21:00, 11 July 2012
no edit summary
#Add DNA/Lipofectamine complexes (~500 uL) to the 2 mL of media on the cells
#Gently rock plate to mix
#After ~4h aspirate media and re-feed cells with normal media. (It is usually possible to leave overnight).
Protocol adapted from [http://tools.invitrogen.com/content/sfs/manuals/lipofectamine2000_man.pdf Product Manual]
Irith
22
edits
Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:MobileDiff/672"

Navigation menu