Changes

==Protocol==* All steps on ice/cold room* Add 50 ul beads (amylose resin #E80021L Biolabs) to 0.5 mg of protein lysate in lysis buffer with protease inhibitors* Add 1 ml of lysis buffer* Rotate in cold room 30’ to ON* Spin down at max speed for 1’* Aspirate supernatant down to 1 mm over beads with crushed tip.* Repeat 3-5 washes with lysis buffer without protease inhibitors * After last wash and aspiration spin again and aspirate all supernatant* Ad 50ul SDS loading buffer, boil for 5 minutes* Load 10ul/gel