903
edits
Changes
updated protocol to make 300 uL of lipid to make extruding easier
==Preparation of Liposomes==
#Combine DOPC + C16 lipid (or DOPC alone) at a ratio of 97:3 and dry under nitrogen (15uL 30 uL PC or 1429.5uL 1 uL PC + 0.5 9 uL PI)#Resuspend to 10 mM total lipid with HBS-N (100 300 uL). Vortex thoroughly and sonicate in water bath#Correct pH to 7.4 using pH paper and 0.6uL aliquots (usually ~ 1uL of 1M NaOH)
#Freeze thaw in a water bath sonicator at 40C and liquid nitrogen 8 times.
#Pass 10X through a polycarbonate filter using an Avanti MiniExtruder at Room Temperature.
