Changes

Jump to: navigation, search

Glycogen Determination from Cells

66 bytes added, 13:17, 6 October 2010
added cell lysis as per alan cheng
==Protocol==
# Treat cells as desired and wash 2x with ice cold PBS.
# Scrape cells into 0.5 mL of Sodium Acetate (for 12 well). Lyse by either brief sonication (~10s) or 3x freeze thaw cycles.
# Measure protein content by bradford assay for normalization
# Quantify glucose using kit:

Navigation menu