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Created page with 'Carboxypeptidase Secretion Assay == Protocol == *Revive Yeast Cells (2-3) days *Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast. (Does this have to be sele...'
Carboxypeptidase Secretion Assay
== Protocol ==
*Revive Yeast Cells (2-3) days
*Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast. (Does this have to be selective plates? or are YPD plates always used?)
*Place nitrocellulose filters on gel and incubate overnight at 23(So you're not actually moving the patch to the filter, but just allowing it to touch?)
*Wash filter in water (by adding water to dish and rocking?)
*Primary antibody (polyclonal against cpy location?) how long?
*Secondary antibody (where/what is it and how long?)
== Protocol ==
*Revive Yeast Cells (2-3) days
*Pick one colony and make a 1/2 inch x 1/2 inch square patch of yeast. (Does this have to be selective plates? or are YPD plates always used?)
*Place nitrocellulose filters on gel and incubate overnight at 23(So you're not actually moving the patch to the filter, but just allowing it to touch?)
*Wash filter in water (by adding water to dish and rocking?)
*Primary antibody (polyclonal against cpy location?) how long?
*Secondary antibody (where/what is it and how long?)
