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# Incubate overnight at room temperature.
# Add lysis buffer and blot using HA antibodies.
==From Park et al==
Log phase cells were resuspended in 1 ml ice cold 0.2 M NaOH containing 0.2 % β-
mercaptoethanol (v/v) and incubated on ice for 10 min. Then 50 μl of trichloroacetic acid
was added and samples were incubated on ice for 10 min. 1M Tris base was added, and
samples were boiled in 2X SDS sample buffer. SDS-PAGE and transfer to nitrocellulose
was performed using standard protocols. Proteins were blotted from a 6% gel and a 4-20% gel
