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→Basic Protocol
# Stimulate cells if necessary (i.e. insulin treatment for 10 min)
# Wash cells 2x1mL (1mL per well for 12 well plates, 2mL per well for 6 well plates) with ice cold PBS -/- and aspirate
# Add 200uL [[Buffer:(RIPA]] buffer ) and scrape cells
# Pipet into cold eppendorf tubes
# rotate end over end for 30 minutes at 4oC to lyse
