9
edits
Changes
→RIPA Buffer (for 10mL lysis buffer)
! || Final Concentration || per 10 mL || Stock
|-
|Tris pH7.4 || 50mM || 500uL || 1M(pH 8.0@25oC)
|-
|Na Deoxycholate || 0.25% || 250uL || 10%
|EDTA || 1mM || 20uL || 0.5M
|-
|NaVO3 || 1mM 100uM || 100uL 10uL || 1M100mM
|-
|NaF || 5mM || 100uL || 250mM0.5M
|-
|NaPPi || 25 mM || 1 mL || 500mM250mM
|-
|- Protease Inhibitors !! 1 mini tab
|}
