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→Protocol
#Warm PBS and Media in water bath
#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-
#Add 1 mL trypsin and sit in the hoodfor 2-5 min
#Add 10 mL media to each new dish
#Check cells for trypsinization, and if necessary tap the cells
#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)
#Replace plates in the incubator
==Cell Specific Notes==
*3T3-L1 fibroblasts have special considerations regarding confluence. See [[Differentiation of 3T3-L1 Cells]]
