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*When splitting cells, wash cells 2x with sterile PBS then add 0.05% trypsin and sit at room temperature for 2-5 min. Be careful not to over-trypsinize cells
*Cells can normally be passaged up to about passage # 25 without problems.
*Plate cells out in whichever format you require your adipocytes in (ie i.e. 12 well plates or 15 cm dishes)
==Differentiation of Fibroblasts to Adipocytes==
