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==Programs Required==
*Pymolhttp://pymol.sourceforge.net/*APBShttp://sourceforge.net/projects/apbs/
===Generate PQR File and PX Files for the Structure===
#Download or generate PDB file.
#Go to the PDB2PQR server at http://pdb2pqr-1.wustl.edu/pdb2pqr/. The default settings (AMBER forcefield, internal naming scheme), are ok. Check off the prepare APBS input file.
#Save the pqr file and the APBS input file.
#Run APBS as '''apbs inputfile.in'''. This will generate a *.dx file.
===Visualizing Electrostatic Surface===
#Open pdb file then dx file in pymol.
#Go to plugin -> apbs tools -> visualization.
#Go to the visualization tab.
#Click on show and adjust settings to -10kV to 10kV and press show again.
[[Category: Structures]]
[[Category: PyMol]]
[[Category: Bioinformatics]]
